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rabbit anti-nep antibody  (Millipore)


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    Structured Review

    Millipore rabbit anti-nep antibody
    Rabbit Anti Nep Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-nep+antibody/rabbit+anti+nep+antibody/pm40268067-137-71-75
    Average 90 stars, based on 1 article reviews
    rabbit anti-nep antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Substance P Promotes Wound Healing in Diabetes by Modulating Inflammation and Macrophage Phenotype
    Article Snippet: IHC IHC-Paraffin Paraffin sections (5 μm) were stained with rabbit anti-NEP antibody (Millipore, Billerica, MA).

    Article Title: Z-Guggulsterone attenuates cognitive defects and decreases neuroinflammation in APPswe/PS1dE9 mice through inhibiting the TLR4 signaling pathway.
    Article Snippet: Growing evidence indicates that inflammatory damage is implicated in the pathogenesis of Alzheimer’s disease (AD).. Z-Guggulsterone (Z-GS) is a natural steroid, which is extracted from Commiphora mukul and has antiinflammatory effects in vivo and in vitro.. In the present study, we investigated the disease-modifying effects of chronic Z-GS administration on the cognitive and neuropathological impairments in the transgenic mouse models of AD.

    Article Title: Locomotor Hyperactivity in the Early-Stage Alzheimer’s Disease-like Pathology of APP/PS1 Mice: Associated with Impaired Polarization of Astrocyte Aquaporin 4
    Article Snippet: After blocking in 5% defatted milk for 1 hour, the membrane was incubated at 4°C overnight with appropriate dilutions of following primary antibodies: rabbit anti-AQP4 antibody (1:400; Millipore; Cat# AB3594), mouse GFAP antibody (1:1000; Millipore, Burlington, USA; Cat# MAB360), rabbit antia disintegrin and metalloproteinase 10 (ADAM10) antibody (1:1000; Millipore; Cat# AB19026), mouse antiβ-site amyloid precursor protein-cleaving enzyme 1 (BACE1) antibody (1:1000; Millipore, #MAB5308), rabbit anti-PS1 antibody (1:1000; Sigma-Aldrich, #PRS4203), rabbit anti-neprilysin (NEP) antibody (1:1000; Millipore, #AB5458), rabbit anti-insulindegrading enzyme (IDE) antibody (1:1000; Abcam, #ab32216), rabbit anti-APP (C-terminal) antibody (1:1000; Sigma-Aldrich, #A8717) or mouse antiglyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:3000; Proteintech; #6004-1-Ig).

    Article Title: Erinacine S, a Rare Sesterterpene from the Mycelia of Hericium erinaceus.
    Article Snippet: A new sesterterpene, erinacine S, and one cyathane diterpene xyloside, erinacine A, were isolated from the ethanol extract of the mycelia of Hericium erinaceus.. Their structures were elucidated by spectroscopic and X-ray analysis.. A 30-day oral course of erinacines A and S attenuated Aβ plaque burden in the brains of 5-month-old female APP/PS1 transgenic mice.

    Article Title: Z-Guggulsterone attenuates cognitive deficits and reduces amyloid-β levels in APPswe/PS1dE9 mice by inhibiting toll-like receptor 4 pathway
    Article Snippet: After blockage of nonspeci c binding sites, the membranes were probed with the following antibodies overnight at 4°C: rabbit anti-TLR4 antibody (1:1,000; Abcam), mouse anti-IκBα antibody (1:1000; Cell Signaling Technology), mouse anti-p-IκBα (Ser32/36) antibody (1:500; Santa Cruz Biotechnology), mouse anti-NF-κB p65 antibody (1:1000; Cell Signaling Technology), rabbit anti-NF-κB pp65 (Ser536) antibody (1:1000; Cell Signaling Technology), mouse anti-APP antibody (1:500; Millipore), mouse anti-human sAPPα antibody (1:100; Immuno-Biological Laboratories), mouse anti-human sAPPβ antibody (1:100; Immuno-Biological Laboratories), rabbit anti-ADAM10 antibody (1:2,000; Abcam), rabbit anti-BACE1 antibody (1:1,000; Abcam), rabbit anti-PS1 antibody (1:500; Abcam), rabbit anti-NEP antibody (1:1,000; Millipore), goat anti-IDE antibody (1:500; Sigma-Aldrich), rabbit anti-SYP antibody (1:1,000; Cell Signaling Technology), rabbit anti-PSD95 antibody (1:500; Abcam), and mouse anti-β-actin antibody (1:5,000; Sigma-Aldrich).

    Article Title: Acetyl-11-keto-β-boswellic acid ameliorates cognitive deficits and reduces amyloid-β levels in APPswe/PS1dE9 mice through antioxidant and anti-inflammatory pathways.
    Article Snippet: Alzheimer's disease (AD) is a complex disease involved oxidative stress and inflammation in its pathogenesis.. Acetyl-11-keto-β-boswellic acid (AKBA) is an active triterpenoid compound from extracts of Boswellia serrata, which has been widely used as an antioxidant and anti-inflammatory agent.. The present study was to determine whether AKBA, a novel candidate, could protect against cognitive and neuropathological impairments in AD.

    Article Title: Blockage of CCL3 with neutralizing antibody reduces neuroinflammation and reverses Alzheimer disease phenotypes.
    Article Snippet: Background: Accumulating evidence indicates that neuroinflammation is involved in the pathogenesis of Alzheimer’s disease (AD).. According to RNA sequencing and quantitative PCR (qPCR), we found that chemokine CCL3 mRNA expression was abnormally upregulated in the brains of AD transgenic mice.. Moreover, the levels of CCL3 in the serum of AD patients were significantly elevated and negatively correlated with their cognitive abilities.

    Article Title: N-Acetylcysteine Prevents 4-Hydroxynonenal- and Amyloid-β-Induced Modification and Inactivation of Neprilysin in SH-SY5Y Cells
    Article Snippet: Antibodies were obtained from the following sources: rabbit anti-HNE and rabbit anti-NEP antibody (Millipore, Billerica, MA); mouse CD10 ectodomain (R&D Systems, Inc., Minneapolis, MN).

    Incubation:

    Article Title: Substance P Promotes Wound Healing in Diabetes by Modulating Inflammation and Macrophage Phenotype
    Article Snippet: IHC IHC-Paraffin Paraffin sections (5 μm) were stained with rabbit anti-NEP antibody (Millipore, Billerica, MA).

    Article Title: Z-Guggulsterone attenuates cognitive defects and decreases neuroinflammation in APPswe/PS1dE9 mice through inhibiting the TLR4 signaling pathway.
    Article Snippet: Growing evidence indicates that inflammatory damage is implicated in the pathogenesis of Alzheimer’s disease (AD).. Z-Guggulsterone (Z-GS) is a natural steroid, which is extracted from Commiphora mukul and has antiinflammatory effects in vivo and in vitro.. In the present study, we investigated the disease-modifying effects of chronic Z-GS administration on the cognitive and neuropathological impairments in the transgenic mouse models of AD.

    Article Title: Locomotor Hyperactivity in the Early-Stage Alzheimer’s Disease-like Pathology of APP/PS1 Mice: Associated with Impaired Polarization of Astrocyte Aquaporin 4
    Article Snippet: After blocking in 5% defatted milk for 1 hour, the membrane was incubated at 4°C overnight with appropriate dilutions of following primary antibodies: rabbit anti-AQP4 antibody (1:400; Millipore; Cat# AB3594), mouse GFAP antibody (1:1000; Millipore, Burlington, USA; Cat# MAB360), rabbit antia disintegrin and metalloproteinase 10 (ADAM10) antibody (1:1000; Millipore; Cat# AB19026), mouse antiβ-site amyloid precursor protein-cleaving enzyme 1 (BACE1) antibody (1:1000; Millipore, #MAB5308), rabbit anti-PS1 antibody (1:1000; Sigma-Aldrich, #PRS4203), rabbit anti-neprilysin (NEP) antibody (1:1000; Millipore, #AB5458), rabbit anti-insulindegrading enzyme (IDE) antibody (1:1000; Abcam, #ab32216), rabbit anti-APP (C-terminal) antibody (1:1000; Sigma-Aldrich, #A8717) or mouse antiglyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:3000; Proteintech; #6004-1-Ig).

    Article Title: Erinacine S, a Rare Sesterterpene from the Mycelia of Hericium erinaceus.
    Article Snippet: A new sesterterpene, erinacine S, and one cyathane diterpene xyloside, erinacine A, were isolated from the ethanol extract of the mycelia of Hericium erinaceus.. Their structures were elucidated by spectroscopic and X-ray analysis.. A 30-day oral course of erinacines A and S attenuated Aβ plaque burden in the brains of 5-month-old female APP/PS1 transgenic mice.

    Article Title: Z-Guggulsterone attenuates cognitive deficits and reduces amyloid-β levels in APPswe/PS1dE9 mice by inhibiting toll-like receptor 4 pathway
    Article Snippet: After blockage of nonspeci c binding sites, the membranes were probed with the following antibodies overnight at 4°C: rabbit anti-TLR4 antibody (1:1,000; Abcam), mouse anti-IκBα antibody (1:1000; Cell Signaling Technology), mouse anti-p-IκBα (Ser32/36) antibody (1:500; Santa Cruz Biotechnology), mouse anti-NF-κB p65 antibody (1:1000; Cell Signaling Technology), rabbit anti-NF-κB pp65 (Ser536) antibody (1:1000; Cell Signaling Technology), mouse anti-APP antibody (1:500; Millipore), mouse anti-human sAPPα antibody (1:100; Immuno-Biological Laboratories), mouse anti-human sAPPβ antibody (1:100; Immuno-Biological Laboratories), rabbit anti-ADAM10 antibody (1:2,000; Abcam), rabbit anti-BACE1 antibody (1:1,000; Abcam), rabbit anti-PS1 antibody (1:500; Abcam), rabbit anti-NEP antibody (1:1,000; Millipore), goat anti-IDE antibody (1:500; Sigma-Aldrich), rabbit anti-SYP antibody (1:1,000; Cell Signaling Technology), rabbit anti-PSD95 antibody (1:500; Abcam), and mouse anti-β-actin antibody (1:5,000; Sigma-Aldrich).

    Article Title: Acetyl-11-keto-β-boswellic acid ameliorates cognitive deficits and reduces amyloid-β levels in APPswe/PS1dE9 mice through antioxidant and anti-inflammatory pathways.
    Article Snippet: Alzheimer's disease (AD) is a complex disease involved oxidative stress and inflammation in its pathogenesis.. Acetyl-11-keto-β-boswellic acid (AKBA) is an active triterpenoid compound from extracts of Boswellia serrata, which has been widely used as an antioxidant and anti-inflammatory agent.. The present study was to determine whether AKBA, a novel candidate, could protect against cognitive and neuropathological impairments in AD.

    Article Title: Blockage of CCL3 with neutralizing antibody reduces neuroinflammation and reverses Alzheimer disease phenotypes.
    Article Snippet: Background: Accumulating evidence indicates that neuroinflammation is involved in the pathogenesis of Alzheimer’s disease (AD).. According to RNA sequencing and quantitative PCR (qPCR), we found that chemokine CCL3 mRNA expression was abnormally upregulated in the brains of AD transgenic mice.. Moreover, the levels of CCL3 in the serum of AD patients were significantly elevated and negatively correlated with their cognitive abilities.

    Article Title: N-Acetylcysteine Prevents 4-Hydroxynonenal- and Amyloid-β-Induced Modification and Inactivation of Neprilysin in SH-SY5Y Cells
    Article Snippet: Antibodies were obtained from the following sources: rabbit anti-HNE and rabbit anti-NEP antibody (Millipore, Billerica, MA); mouse CD10 ectodomain (R&D Systems, Inc., Minneapolis, MN).

    Blocking Assay:

    Article Title: Substance P Promotes Wound Healing in Diabetes by Modulating Inflammation and Macrophage Phenotype
    Article Snippet: IHC IHC-Paraffin Paraffin sections (5 μm) were stained with rabbit anti-NEP antibody (Millipore, Billerica, MA).

    Article Title: Z-Guggulsterone attenuates cognitive defects and decreases neuroinflammation in APPswe/PS1dE9 mice through inhibiting the TLR4 signaling pathway.
    Article Snippet: Growing evidence indicates that inflammatory damage is implicated in the pathogenesis of Alzheimer’s disease (AD).. Z-Guggulsterone (Z-GS) is a natural steroid, which is extracted from Commiphora mukul and has antiinflammatory effects in vivo and in vitro.. In the present study, we investigated the disease-modifying effects of chronic Z-GS administration on the cognitive and neuropathological impairments in the transgenic mouse models of AD.

    Article Title: Locomotor Hyperactivity in the Early-Stage Alzheimer’s Disease-like Pathology of APP/PS1 Mice: Associated with Impaired Polarization of Astrocyte Aquaporin 4
    Article Snippet: After blocking in 5% defatted milk for 1 hour, the membrane was incubated at 4°C overnight with appropriate dilutions of following primary antibodies: rabbit anti-AQP4 antibody (1:400; Millipore; Cat# AB3594), mouse GFAP antibody (1:1000; Millipore, Burlington, USA; Cat# MAB360), rabbit antia disintegrin and metalloproteinase 10 (ADAM10) antibody (1:1000; Millipore; Cat# AB19026), mouse antiβ-site amyloid precursor protein-cleaving enzyme 1 (BACE1) antibody (1:1000; Millipore, #MAB5308), rabbit anti-PS1 antibody (1:1000; Sigma-Aldrich, #PRS4203), rabbit anti-neprilysin (NEP) antibody (1:1000; Millipore, #AB5458), rabbit anti-insulindegrading enzyme (IDE) antibody (1:1000; Abcam, #ab32216), rabbit anti-APP (C-terminal) antibody (1:1000; Sigma-Aldrich, #A8717) or mouse antiglyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:3000; Proteintech; #6004-1-Ig).

    Article Title: Erinacine S, a Rare Sesterterpene from the Mycelia of Hericium erinaceus.
    Article Snippet: A new sesterterpene, erinacine S, and one cyathane diterpene xyloside, erinacine A, were isolated from the ethanol extract of the mycelia of Hericium erinaceus.. Their structures were elucidated by spectroscopic and X-ray analysis.. A 30-day oral course of erinacines A and S attenuated Aβ plaque burden in the brains of 5-month-old female APP/PS1 transgenic mice.

    Article Title: Z-Guggulsterone attenuates cognitive deficits and reduces amyloid-β levels in APPswe/PS1dE9 mice by inhibiting toll-like receptor 4 pathway
    Article Snippet: After blockage of nonspeci c binding sites, the membranes were probed with the following antibodies overnight at 4°C: rabbit anti-TLR4 antibody (1:1,000; Abcam), mouse anti-IκBα antibody (1:1000; Cell Signaling Technology), mouse anti-p-IκBα (Ser32/36) antibody (1:500; Santa Cruz Biotechnology), mouse anti-NF-κB p65 antibody (1:1000; Cell Signaling Technology), rabbit anti-NF-κB pp65 (Ser536) antibody (1:1000; Cell Signaling Technology), mouse anti-APP antibody (1:500; Millipore), mouse anti-human sAPPα antibody (1:100; Immuno-Biological Laboratories), mouse anti-human sAPPβ antibody (1:100; Immuno-Biological Laboratories), rabbit anti-ADAM10 antibody (1:2,000; Abcam), rabbit anti-BACE1 antibody (1:1,000; Abcam), rabbit anti-PS1 antibody (1:500; Abcam), rabbit anti-NEP antibody (1:1,000; Millipore), goat anti-IDE antibody (1:500; Sigma-Aldrich), rabbit anti-SYP antibody (1:1,000; Cell Signaling Technology), rabbit anti-PSD95 antibody (1:500; Abcam), and mouse anti-β-actin antibody (1:5,000; Sigma-Aldrich).

    Article Title: Acetyl-11-keto-β-boswellic acid ameliorates cognitive deficits and reduces amyloid-β levels in APPswe/PS1dE9 mice through antioxidant and anti-inflammatory pathways.
    Article Snippet: Alzheimer's disease (AD) is a complex disease involved oxidative stress and inflammation in its pathogenesis.. Acetyl-11-keto-β-boswellic acid (AKBA) is an active triterpenoid compound from extracts of Boswellia serrata, which has been widely used as an antioxidant and anti-inflammatory agent.. The present study was to determine whether AKBA, a novel candidate, could protect against cognitive and neuropathological impairments in AD.

    Article Title: Blockage of CCL3 with neutralizing antibody reduces neuroinflammation and reverses Alzheimer disease phenotypes.
    Article Snippet: Background: Accumulating evidence indicates that neuroinflammation is involved in the pathogenesis of Alzheimer’s disease (AD).. According to RNA sequencing and quantitative PCR (qPCR), we found that chemokine CCL3 mRNA expression was abnormally upregulated in the brains of AD transgenic mice.. Moreover, the levels of CCL3 in the serum of AD patients were significantly elevated and negatively correlated with their cognitive abilities.

    Article Title: N-Acetylcysteine Prevents 4-Hydroxynonenal- and Amyloid-β-Induced Modification and Inactivation of Neprilysin in SH-SY5Y Cells
    Article Snippet: Antibodies were obtained from the following sources: rabbit anti-HNE and rabbit anti-NEP antibody (Millipore, Billerica, MA); mouse CD10 ectodomain (R&D Systems, Inc., Minneapolis, MN).

    Membrane:

    Article Title: Substance P Promotes Wound Healing in Diabetes by Modulating Inflammation and Macrophage Phenotype
    Article Snippet: IHC IHC-Paraffin Paraffin sections (5 μm) were stained with rabbit anti-NEP antibody (Millipore, Billerica, MA).

    Article Title: Z-Guggulsterone attenuates cognitive defects and decreases neuroinflammation in APPswe/PS1dE9 mice through inhibiting the TLR4 signaling pathway.
    Article Snippet: Growing evidence indicates that inflammatory damage is implicated in the pathogenesis of Alzheimer’s disease (AD).. Z-Guggulsterone (Z-GS) is a natural steroid, which is extracted from Commiphora mukul and has antiinflammatory effects in vivo and in vitro.. In the present study, we investigated the disease-modifying effects of chronic Z-GS administration on the cognitive and neuropathological impairments in the transgenic mouse models of AD.

    Article Title: Locomotor Hyperactivity in the Early-Stage Alzheimer’s Disease-like Pathology of APP/PS1 Mice: Associated with Impaired Polarization of Astrocyte Aquaporin 4
    Article Snippet: After blocking in 5% defatted milk for 1 hour, the membrane was incubated at 4°C overnight with appropriate dilutions of following primary antibodies: rabbit anti-AQP4 antibody (1:400; Millipore; Cat# AB3594), mouse GFAP antibody (1:1000; Millipore, Burlington, USA; Cat# MAB360), rabbit antia disintegrin and metalloproteinase 10 (ADAM10) antibody (1:1000; Millipore; Cat# AB19026), mouse antiβ-site amyloid precursor protein-cleaving enzyme 1 (BACE1) antibody (1:1000; Millipore, #MAB5308), rabbit anti-PS1 antibody (1:1000; Sigma-Aldrich, #PRS4203), rabbit anti-neprilysin (NEP) antibody (1:1000; Millipore, #AB5458), rabbit anti-insulindegrading enzyme (IDE) antibody (1:1000; Abcam, #ab32216), rabbit anti-APP (C-terminal) antibody (1:1000; Sigma-Aldrich, #A8717) or mouse antiglyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:3000; Proteintech; #6004-1-Ig).

    Article Title: Erinacine S, a Rare Sesterterpene from the Mycelia of Hericium erinaceus.
    Article Snippet: A new sesterterpene, erinacine S, and one cyathane diterpene xyloside, erinacine A, were isolated from the ethanol extract of the mycelia of Hericium erinaceus.. Their structures were elucidated by spectroscopic and X-ray analysis.. A 30-day oral course of erinacines A and S attenuated Aβ plaque burden in the brains of 5-month-old female APP/PS1 transgenic mice.

    Article Title: Z-Guggulsterone attenuates cognitive deficits and reduces amyloid-β levels in APPswe/PS1dE9 mice by inhibiting toll-like receptor 4 pathway
    Article Snippet: After blockage of nonspeci c binding sites, the membranes were probed with the following antibodies overnight at 4°C: rabbit anti-TLR4 antibody (1:1,000; Abcam), mouse anti-IκBα antibody (1:1000; Cell Signaling Technology), mouse anti-p-IκBα (Ser32/36) antibody (1:500; Santa Cruz Biotechnology), mouse anti-NF-κB p65 antibody (1:1000; Cell Signaling Technology), rabbit anti-NF-κB pp65 (Ser536) antibody (1:1000; Cell Signaling Technology), mouse anti-APP antibody (1:500; Millipore), mouse anti-human sAPPα antibody (1:100; Immuno-Biological Laboratories), mouse anti-human sAPPβ antibody (1:100; Immuno-Biological Laboratories), rabbit anti-ADAM10 antibody (1:2,000; Abcam), rabbit anti-BACE1 antibody (1:1,000; Abcam), rabbit anti-PS1 antibody (1:500; Abcam), rabbit anti-NEP antibody (1:1,000; Millipore), goat anti-IDE antibody (1:500; Sigma-Aldrich), rabbit anti-SYP antibody (1:1,000; Cell Signaling Technology), rabbit anti-PSD95 antibody (1:500; Abcam), and mouse anti-β-actin antibody (1:5,000; Sigma-Aldrich).

    Article Title: Acetyl-11-keto-β-boswellic acid ameliorates cognitive deficits and reduces amyloid-β levels in APPswe/PS1dE9 mice through antioxidant and anti-inflammatory pathways.
    Article Snippet: Alzheimer's disease (AD) is a complex disease involved oxidative stress and inflammation in its pathogenesis.. Acetyl-11-keto-β-boswellic acid (AKBA) is an active triterpenoid compound from extracts of Boswellia serrata, which has been widely used as an antioxidant and anti-inflammatory agent.. The present study was to determine whether AKBA, a novel candidate, could protect against cognitive and neuropathological impairments in AD.

    Article Title: Blockage of CCL3 with neutralizing antibody reduces neuroinflammation and reverses Alzheimer disease phenotypes.
    Article Snippet: Background: Accumulating evidence indicates that neuroinflammation is involved in the pathogenesis of Alzheimer’s disease (AD).. According to RNA sequencing and quantitative PCR (qPCR), we found that chemokine CCL3 mRNA expression was abnormally upregulated in the brains of AD transgenic mice.. Moreover, the levels of CCL3 in the serum of AD patients were significantly elevated and negatively correlated with their cognitive abilities.

    Article Title: N-Acetylcysteine Prevents 4-Hydroxynonenal- and Amyloid-β-Induced Modification and Inactivation of Neprilysin in SH-SY5Y Cells
    Article Snippet: Antibodies were obtained from the following sources: rabbit anti-HNE and rabbit anti-NEP antibody (Millipore, Billerica, MA); mouse CD10 ectodomain (R&D Systems, Inc., Minneapolis, MN).

    Immunohistochemistry:

    Article Title: Substance P Promotes Wound Healing in Diabetes by Modulating Inflammation and Macrophage Phenotype
    Article Snippet: IHC IHC-Paraffin Paraffin sections (5 μm) were stained with rabbit anti-NEP antibody (Millipore, Billerica, MA).

    Article Title: Z-Guggulsterone attenuates cognitive defects and decreases neuroinflammation in APPswe/PS1dE9 mice through inhibiting the TLR4 signaling pathway.
    Article Snippet: Growing evidence indicates that inflammatory damage is implicated in the pathogenesis of Alzheimer’s disease (AD).. Z-Guggulsterone (Z-GS) is a natural steroid, which is extracted from Commiphora mukul and has antiinflammatory effects in vivo and in vitro.. In the present study, we investigated the disease-modifying effects of chronic Z-GS administration on the cognitive and neuropathological impairments in the transgenic mouse models of AD.

    Article Title: Locomotor Hyperactivity in the Early-Stage Alzheimer’s Disease-like Pathology of APP/PS1 Mice: Associated with Impaired Polarization of Astrocyte Aquaporin 4
    Article Snippet: After blocking in 5% defatted milk for 1 hour, the membrane was incubated at 4°C overnight with appropriate dilutions of following primary antibodies: rabbit anti-AQP4 antibody (1:400; Millipore; Cat# AB3594), mouse GFAP antibody (1:1000; Millipore, Burlington, USA; Cat# MAB360), rabbit antia disintegrin and metalloproteinase 10 (ADAM10) antibody (1:1000; Millipore; Cat# AB19026), mouse antiβ-site amyloid precursor protein-cleaving enzyme 1 (BACE1) antibody (1:1000; Millipore, #MAB5308), rabbit anti-PS1 antibody (1:1000; Sigma-Aldrich, #PRS4203), rabbit anti-neprilysin (NEP) antibody (1:1000; Millipore, #AB5458), rabbit anti-insulindegrading enzyme (IDE) antibody (1:1000; Abcam, #ab32216), rabbit anti-APP (C-terminal) antibody (1:1000; Sigma-Aldrich, #A8717) or mouse antiglyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:3000; Proteintech; #6004-1-Ig).

    Article Title: Erinacine S, a Rare Sesterterpene from the Mycelia of Hericium erinaceus.
    Article Snippet: A new sesterterpene, erinacine S, and one cyathane diterpene xyloside, erinacine A, were isolated from the ethanol extract of the mycelia of Hericium erinaceus.. Their structures were elucidated by spectroscopic and X-ray analysis.. A 30-day oral course of erinacines A and S attenuated Aβ plaque burden in the brains of 5-month-old female APP/PS1 transgenic mice.

    Article Title: Z-Guggulsterone attenuates cognitive deficits and reduces amyloid-β levels in APPswe/PS1dE9 mice by inhibiting toll-like receptor 4 pathway
    Article Snippet: After blockage of nonspeci c binding sites, the membranes were probed with the following antibodies overnight at 4°C: rabbit anti-TLR4 antibody (1:1,000; Abcam), mouse anti-IκBα antibody (1:1000; Cell Signaling Technology), mouse anti-p-IκBα (Ser32/36) antibody (1:500; Santa Cruz Biotechnology), mouse anti-NF-κB p65 antibody (1:1000; Cell Signaling Technology), rabbit anti-NF-κB pp65 (Ser536) antibody (1:1000; Cell Signaling Technology), mouse anti-APP antibody (1:500; Millipore), mouse anti-human sAPPα antibody (1:100; Immuno-Biological Laboratories), mouse anti-human sAPPβ antibody (1:100; Immuno-Biological Laboratories), rabbit anti-ADAM10 antibody (1:2,000; Abcam), rabbit anti-BACE1 antibody (1:1,000; Abcam), rabbit anti-PS1 antibody (1:500; Abcam), rabbit anti-NEP antibody (1:1,000; Millipore), goat anti-IDE antibody (1:500; Sigma-Aldrich), rabbit anti-SYP antibody (1:1,000; Cell Signaling Technology), rabbit anti-PSD95 antibody (1:500; Abcam), and mouse anti-β-actin antibody (1:5,000; Sigma-Aldrich).

    Article Title: Acetyl-11-keto-β-boswellic acid ameliorates cognitive deficits and reduces amyloid-β levels in APPswe/PS1dE9 mice through antioxidant and anti-inflammatory pathways.
    Article Snippet: Alzheimer's disease (AD) is a complex disease involved oxidative stress and inflammation in its pathogenesis.. Acetyl-11-keto-β-boswellic acid (AKBA) is an active triterpenoid compound from extracts of Boswellia serrata, which has been widely used as an antioxidant and anti-inflammatory agent.. The present study was to determine whether AKBA, a novel candidate, could protect against cognitive and neuropathological impairments in AD.

    Article Title: Blockage of CCL3 with neutralizing antibody reduces neuroinflammation and reverses Alzheimer disease phenotypes.
    Article Snippet: Background: Accumulating evidence indicates that neuroinflammation is involved in the pathogenesis of Alzheimer’s disease (AD).. According to RNA sequencing and quantitative PCR (qPCR), we found that chemokine CCL3 mRNA expression was abnormally upregulated in the brains of AD transgenic mice.. Moreover, the levels of CCL3 in the serum of AD patients were significantly elevated and negatively correlated with their cognitive abilities.

    Article Title: N-Acetylcysteine Prevents 4-Hydroxynonenal- and Amyloid-β-Induced Modification and Inactivation of Neprilysin in SH-SY5Y Cells
    Article Snippet: Antibodies were obtained from the following sources: rabbit anti-HNE and rabbit anti-NEP antibody (Millipore, Billerica, MA); mouse CD10 ectodomain (R&D Systems, Inc., Minneapolis, MN).

    Staining:

    Article Title: Substance P Promotes Wound Healing in Diabetes by Modulating Inflammation and Macrophage Phenotype
    Article Snippet: IHC IHC-Paraffin Paraffin sections (5 μm) were stained with rabbit anti-NEP antibody (Millipore, Billerica, MA).

    Article Title: Z-Guggulsterone attenuates cognitive defects and decreases neuroinflammation in APPswe/PS1dE9 mice through inhibiting the TLR4 signaling pathway.
    Article Snippet: Growing evidence indicates that inflammatory damage is implicated in the pathogenesis of Alzheimer’s disease (AD).. Z-Guggulsterone (Z-GS) is a natural steroid, which is extracted from Commiphora mukul and has antiinflammatory effects in vivo and in vitro.. In the present study, we investigated the disease-modifying effects of chronic Z-GS administration on the cognitive and neuropathological impairments in the transgenic mouse models of AD.

    Article Title: Locomotor Hyperactivity in the Early-Stage Alzheimer’s Disease-like Pathology of APP/PS1 Mice: Associated with Impaired Polarization of Astrocyte Aquaporin 4
    Article Snippet: After blocking in 5% defatted milk for 1 hour, the membrane was incubated at 4°C overnight with appropriate dilutions of following primary antibodies: rabbit anti-AQP4 antibody (1:400; Millipore; Cat# AB3594), mouse GFAP antibody (1:1000; Millipore, Burlington, USA; Cat# MAB360), rabbit antia disintegrin and metalloproteinase 10 (ADAM10) antibody (1:1000; Millipore; Cat# AB19026), mouse antiβ-site amyloid precursor protein-cleaving enzyme 1 (BACE1) antibody (1:1000; Millipore, #MAB5308), rabbit anti-PS1 antibody (1:1000; Sigma-Aldrich, #PRS4203), rabbit anti-neprilysin (NEP) antibody (1:1000; Millipore, #AB5458), rabbit anti-insulindegrading enzyme (IDE) antibody (1:1000; Abcam, #ab32216), rabbit anti-APP (C-terminal) antibody (1:1000; Sigma-Aldrich, #A8717) or mouse antiglyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:3000; Proteintech; #6004-1-Ig).

    Article Title: Erinacine S, a Rare Sesterterpene from the Mycelia of Hericium erinaceus.
    Article Snippet: A new sesterterpene, erinacine S, and one cyathane diterpene xyloside, erinacine A, were isolated from the ethanol extract of the mycelia of Hericium erinaceus.. Their structures were elucidated by spectroscopic and X-ray analysis.. A 30-day oral course of erinacines A and S attenuated Aβ plaque burden in the brains of 5-month-old female APP/PS1 transgenic mice.

    Article Title: Z-Guggulsterone attenuates cognitive deficits and reduces amyloid-β levels in APPswe/PS1dE9 mice by inhibiting toll-like receptor 4 pathway
    Article Snippet: After blockage of nonspeci c binding sites, the membranes were probed with the following antibodies overnight at 4°C: rabbit anti-TLR4 antibody (1:1,000; Abcam), mouse anti-IκBα antibody (1:1000; Cell Signaling Technology), mouse anti-p-IκBα (Ser32/36) antibody (1:500; Santa Cruz Biotechnology), mouse anti-NF-κB p65 antibody (1:1000; Cell Signaling Technology), rabbit anti-NF-κB pp65 (Ser536) antibody (1:1000; Cell Signaling Technology), mouse anti-APP antibody (1:500; Millipore), mouse anti-human sAPPα antibody (1:100; Immuno-Biological Laboratories), mouse anti-human sAPPβ antibody (1:100; Immuno-Biological Laboratories), rabbit anti-ADAM10 antibody (1:2,000; Abcam), rabbit anti-BACE1 antibody (1:1,000; Abcam), rabbit anti-PS1 antibody (1:500; Abcam), rabbit anti-NEP antibody (1:1,000; Millipore), goat anti-IDE antibody (1:500; Sigma-Aldrich), rabbit anti-SYP antibody (1:1,000; Cell Signaling Technology), rabbit anti-PSD95 antibody (1:500; Abcam), and mouse anti-β-actin antibody (1:5,000; Sigma-Aldrich).

    Article Title: Acetyl-11-keto-β-boswellic acid ameliorates cognitive deficits and reduces amyloid-β levels in APPswe/PS1dE9 mice through antioxidant and anti-inflammatory pathways.
    Article Snippet: Alzheimer's disease (AD) is a complex disease involved oxidative stress and inflammation in its pathogenesis.. Acetyl-11-keto-β-boswellic acid (AKBA) is an active triterpenoid compound from extracts of Boswellia serrata, which has been widely used as an antioxidant and anti-inflammatory agent.. The present study was to determine whether AKBA, a novel candidate, could protect against cognitive and neuropathological impairments in AD.

    Article Title: Blockage of CCL3 with neutralizing antibody reduces neuroinflammation and reverses Alzheimer disease phenotypes.
    Article Snippet: Background: Accumulating evidence indicates that neuroinflammation is involved in the pathogenesis of Alzheimer’s disease (AD).. According to RNA sequencing and quantitative PCR (qPCR), we found that chemokine CCL3 mRNA expression was abnormally upregulated in the brains of AD transgenic mice.. Moreover, the levels of CCL3 in the serum of AD patients were significantly elevated and negatively correlated with their cognitive abilities.

    Article Title: N-Acetylcysteine Prevents 4-Hydroxynonenal- and Amyloid-β-Induced Modification and Inactivation of Neprilysin in SH-SY5Y Cells
    Article Snippet: Antibodies were obtained from the following sources: rabbit anti-HNE and rabbit anti-NEP antibody (Millipore, Billerica, MA); mouse CD10 ectodomain (R&D Systems, Inc., Minneapolis, MN).



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